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Image Search Results
Journal: Scientific Reports
Article Title: Transcriptional activation of follistatin by Nrf2 protects pulmonary epithelial cells against silica nanoparticle-induced oxidative stress
doi: 10.1038/srep21133
Figure Lengend Snippet: ( A,B ) A549 cells were transfected with siRNA targeting FST (siFST) or control siRNA (siCon). The FST mRNA level was detected by qPCR ( A ) and the FST protein level was detected by immunoblotting ( B–E ) A549 cells transfected with siRNA targeting FST (siFST) or control siRNA (siCon) were treated with or without 50 μg/ml of SiO 2 NPs for 12 h. ( C ) Cell viability was determined using cell counting kit-8 (CCK-8). ( D ) Cell apoptosis was monitored by annexin V-FITC staining and flow cytometry analysis. ( E ) Annexin-V positive cells were quantified. The values are the mean ± SD of three independent experiments. ** P < 0.01.
Article Snippet: Briefly, resuspended cells were stained with 5 μl of
Techniques: Transfection, Control, Western Blot, Cell Counting, CCK-8 Assay, Staining, Flow Cytometry